An Integrated Analysis of Transcriptome and miRNA Sequencing Provides Insights into the Dynamic Regulations during Flower Morphogenesis in Petunia

Published genome sequences can facilitate multiple sequencing studies of flower development, which serve as the basis for later analysis variation in phenotypes. To identify potential regulators related to morphology, we captured dynamic expression patterns under five different developmental stages petunia flowers, a popular bedding plant, using transcriptome and miRNA sequencing. The significant transcription factor (TF) families, including MYB, MADS, bHLH, were elucidated. MADS-box genes exhibited co-expression with BBR-BPC, GATA, Dof modules according weighted gene network analysis. Through sequencing, total 45 conserved 26 novel miRNAs identified. According GO KEGG enrichment analysis, carbohydrate metabolic process, photosynthesis, phenylalanine metabolism at transcriptomic level, while response hormone pathways was significantly enriched by DEmiR-targeted genes. Finally, an miRNA–RNA constructed, suggested possibility miRNA-mediated regulation being activated during development. Overall, data present study provide insights into regulatory facilitated TFs, miRNA, their target